In vitro activity of Albizia gummifera (J.F. Gmel.) C.A. Sm. seed extract against promastigote stages of five Leishmania species known to cause human leishmaniasis

Authors

  • Kidist Zealiyas Ethiopian Public Health Institute Author
  • Geremew Tasew Ethiopian Public Health Institute Author
  • Yonas Wuletaw Ethiopian Public Health Institute Author
  • Asfaw Debella Ethiopian Public Health Institute Author
  • Kissi Mudie Ethiopian Public Health Institute Author
  • Getachew Addis Ethiopian Public Health Institute Author
  • Abraham Ali Ethiopian Public Health Institute Author
  • Asrat Hailu Addis Ababa University Author
  • Beyene Petros Addis Ababa University Author
  • Amha Kebede Ethiopian Public Health Institute Author

Keywords:

anti-leishmanial, lead compound(s), crude extract, n-butanol, LC50, IC50 values

Abstract

Background׃ Modern drugs for treatments of leishmaniasis are expensive, bearing limited efficacy and significant toxicity as well as emergence of resistant parasite to them. Several medicinal plants are being used traditionally to treat Leishmania infections in Ethiopia. Therefore the discovery of safer and more efficacious drug from natural product is so essential.

Objective: To investigate the antipromastigote activity of crude extract of Albizia gummifera seed against five Leishmania species responsible to cause cutaneous and visceral leishmaniasis.

Methods: The plant materials were macerated and extracted using 70% ethanol. The extract of each plant was screened for its antipromastigote and hemolytic activities in vitro. Data analysis was done by using Graphpad Prism version 6 software. The criterion for activity was considered as an IC50<100 μg/ml. 

Results:  Haemolytic test of ethanol extracts A. gummifera showed LC50 value of 453.55 ±3.9 µg/ml. The crude ethanol extract of A. gummifera showed the highest potency against promastigotes of L. tropica, L. major, L. donovani, L. chagasi and L. aethiopica species with IC50 values less than10 μg/ml. Among the different fractions of A. gummifera, the n-butanol fraction showed comparable anti-leishmanial activity (IC50 ranges between 0.18 and 0.28 µg/ml) with the standard drug, Amphotericin   B, having IC50 between 0.24 and 0.29 µg/ml.

Conclusions: A. gummifera seed extract considered as a good candidate for further bioassay-guided fractionation and isolation of anti-leishmanial lead compound(s) that could be used for anti-leishmanial drug discovery.

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Author Biographies

  • Kidist Zealiyas, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia 

  • Geremew Tasew, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia 

  • Yonas Wuletaw, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia

  • Asfaw Debella, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia 

  • Kissi Mudie, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia 

  • Getachew Addis, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia 

  • Abraham Ali, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia 

  • Asrat Hailu, Addis Ababa University

    Addis Ababa University, Department of Microbial, Cellular and Molecular Biology. P.O.Box 1176

  • Beyene Petros, Addis Ababa University

    Addis Ababa University, Department of Microbiology, Immunology and Parasitology, P.O.Box 9086

  • Amha Kebede, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, P.O.BOX 1242, Addis Ababa, Ethiopia

References

Ashford, RW., Bray, MA., Hutchinson, MP. & Bray, RS. (1973). The epidemiology of cutaneous leishmaniasis in Ethiopia. Transactions of the Royal Society of Tropical Medicine and Hygiene, 67, 569-601.

Bero, J., Hannaert, V., Chataigné, G., Hérent, MF. & Quetin-Leclercq, J. (2011). In vitro antitrypanosomal and antileishmanial activity of plants used in Benin in traditional medicine and bioassay-guided fractionation of the most active extract. Journal of Ethnopharmacology, 137, 998–1002.

Croft, SL., Barrett, MP. & Urbina, JA. (2005). Chemotherapy of trypanosomiases and leishmaniasis. Trends in Parasitology, 21, 508-512.

Croft, SL. & Yardley, V. (2002). Chemotherapy of leishmaniasis. Current Pharmaceutical Design, 8, 319–342.

Debella, A. (2002). Manual for phytochemical screening of medicinal Plants, Ethiopian Health and Nutrition Research Institute, Addis Ababa, url: http://idosi.org/mejsr/mejsr8(3)11/6.pdf.

Debella, A., Taye, A., Abebe, D., Mudi, K., Melaku, D. & Taye, G. (2007). Screening of some Ethiopian medicinal plants for mosquito larvicidal effects and phytochemical constituents. Pharmacologyonline, 3, 231–243.

Desjeux, P. (2004). Leishmaniasis: Current situation and new perspectives. Comparative Immunology, Microbiology and Infectious Diseases, 27, 305–318.

FMoH (2013). Guideline for diagnosis, treatment and prevention of: Leishmaniasis in Ethiopia. Federal Ministry of Health, 2nd edition,Addis Ababa.

Frezard, F., Demicheli, C., Ribeiro, RR. & Frézard, F. (2009). Pentavalent antimonials: new perspectives for old drugs, Molecules, 14, 2317–2336.

Ghebreselassie, D., Mekonnen, Y., Gebru, G., Ergete, W. & Huruy, K. (2011). The effects of Moringa stenopetala on blood parameters and histopathology of liver and kidney in mice. Ethiopian Journal of Health Development, 25, 51–57.

Hailu, A., Gebre-Michael, T. & Berhe, NMB. (2006). Leishmaniasis. In: Berhane Y., Haile-Mariam D. & Kloos, H. (Editors). The epidimplogy and ecology of health and diseases in Ethiopia., Sa Books, Addis Ababa, Ethiopia.

Handman, E. (2001). Leishmaniasis: Current status of vaccine development. Clinical Microbiology Reviews, 14, 229–243.

Mikus, J. & Steverding, D. (2000). A simple colorimetric method to screen drug cytotoxicity against Leishmania using the dye Alamar Blue. Parasitology International, 48, 265-267.

Muhammad, I., Midiwo, J., Tekwani, B., Samoylenko, V., Sahu, R., Machumi, F. et al. (2011). Antileishmanial Activity of Kenyan Medicinal Plants. Planta Medica, 77; Congress Abstract, pp.47.

Tiuman, TS., Ueda-Nakamura, T., Dias Filho, BP., Cortez, DAG. & Nakamura, CV. (2005). Studies on the effectiveness of Tanacetum parthenium against Leishmania amazonensis. Acta Protozoologica, 44, 245–251.

WHO (2010). Control of the leishmaniases. WHO Technical Report Series, No.949, Geneva.

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Published

2023-11-16

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Original Article

How to Cite

Zealiyas, K. (2023) “In vitro activity of Albizia gummifera (J.F. Gmel.) C.A. Sm. seed extract against promastigote stages of five Leishmania species known to cause human leishmaniasis ”, Ethiopian Journal of Public Health and Nutrition (EJPHN), 1(1), pp. 41–45. Available at: https://ejphn.ephi.gov.et/index.php/ejphn/article/view/26 (Accessed: 21 May 2024).

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