Evaluation of medicinal plants blend formulation for symptomatic relief, phytochemical and microbiological quality and safety
DOI:
https://doi.org/10.20372/ejphn.v6i1.355Keywords:
Medicinal plants, herbal preparations, symptomatic relief, microbial qualityAbstract
Background: Medicinal plants are employed for the primary health care by the community as a supportive care for symptomatic relief of generalized pain, headache, fever, cough and others symptoms. This study was therefore aimed to evaluate blends of traditionally used medicinal plants for symptomatic relief effects besides determining the microbial and, phytochemical qualities and sensorial acceptability.
Methods: Laboratory animals-based experiment was employed in this study. The medicinal herbs blend preparations used as tea infusion were Cymbopgon citratus, Menta spicata, Thymus schimperi, Ruta chalepensis and Stevia rebaudiana. The essential oil blends of Rosmarinus officinalis, Salvia officinalis and Cymbopgon citratus in aqueous solution were used as oral gargle. The tea infusion and essential oils were administered as a single dose by oral gavage in mice. The doses used for analgesics effect of tea infusion were 350, 700 and 1400mg/Kg, and for the antipyretic effect of the gargle solution was five drops essential oil blends in 100ml distilled water. Oral gargle containing 2100mg/Kg tea infusion and five drops of essential oil in 100ml distilled water were used to evaluate the anti-inflammatory activity in mice. The phytochemical and microbial quality and safety, and sensory acceptability were also investigated for medicinal plant blend preparations. Results were summarized as mean ± standard error of mean. The analysis was done using one-way analysis of variance (ANOVA). The means values were compared using Dunnet’s t- test.
Results: The medicinal herb plant possessed analgesic, anti-pyretic and anti-inflammatory activities. The presence of bioactive compounds that was attributed for symptomatic relief was indicated by quantitative determination of total flavonoids and phenolic compounds in the tea infusion and oral gargle solution. The results also showed that the microbial quality was within prescribed limits of international pharmacopeia and WHO requirements. The tea infusion and oral gargle had good sensory acceptability indicating accepted palatability.
Conclusion: The investigation verified the symptomatic relief effects of the medicinal plant blends besides meeting the phytochemical and microbial quality parameters requirements of the medicinal herb’s blends. The findings entails that the plants may be used to relieve pain, fever and inflammation.
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