Larvicidal activities of column chromatographic fractions of an ethanol leaf extract from Ricinus communis against Anopheles arabiensis Patton (Diptera: Culicidae)


  • Araya Eukubay Ethiopian Public Health Institute Author
  • Emana Getu Addis Ababa University Author
  • Eyob Debebe Ethiopian Public Health Institute Author
  • Mamuye Hadis Ethiopian Public Health Institute Author



Anopheles arabiensis, larvicidal activities, plant extract, Ricinus communis, column chromatography


Background: Long-term use of insecticide-based interventions for malaria control has resulted in the development of behavioral, physiological, and genetic resistance of the Anopheles mosquitoes. This calls for the development of sustainable, effective, and eco-friendly vector control measures. This study aimed to evaluate the bioactivity of column chromatographic fractions of ethanol leaf extract of Ricinus communis against Anopheles arabiensis larvae.

Methods: Dried leaves of the test plant were ground to powder and subjected to maceration using 80% ethanol for 72 hours. The crude extract was further fractionated with column chromatography using solvent systems of gradually increasing polarity. All the fractions were analyzed with thin-layer chromatography. Eluents having similar constituents were pooled and tested for their larvicidal potential against laboratory-reared susceptible An. arabiensis at a concentration ranging from 50ppm to 250ppm following WHO protocol.

Results: Fractions F1, F2, F3, and F5 demonstrated larval mortalities ranging from 34.67% to 100% in all the test concentrations while no larval mortalities were observed for fractions F9, F10, and F11 in all the test concentrations. The highest (100%) larval mortalities were observed for F2 and F3 at 100 to 250 ppm with the LC50 of 21.012 and 28.410 ppm, respectively.

Conclusion: Fractions F2 and F3 caused 100% larval mortality at 100 to 250 ppm concentration with the lowest LC50 of 21.012 and 28.410 ppm, respectively, suggesting their inputs for the development of botanical insecticides.


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Author Biographies

  • Araya Eukubay, Ethiopian Public Health Institute

     Ethiopian Public Health Institute, Addis Ababa, Ethiopia

    Email:           Phone number: +251920868300/75337101

  • Emana Getu, Addis Ababa University

    Department of Zoological Sciences, Addis Ababa University, Addis Ababa, Ethiopia

  • Eyob Debebe, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, Addis Ababa, Ethiopia

  • Mamuye Hadis, Ethiopian Public Health Institute

    Ethiopian Public Health Institute, Addis Ababa, Ethiopia


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Original Article

How to Cite

Eukubay, A. (2023) “Larvicidal activities of column chromatographic fractions of an ethanol leaf extract from Ricinus communis against Anopheles arabiensis Patton (Diptera: Culicidae)”, Ethiopian Journal of Public Health and Nutrition (EJPHN), 6(1), pp. 16–20. doi:10.20372/ejphn.v6i1.357.

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